ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of propofol on myelin basic protein (MBP) expression in oligodendrocytes of SD rats at different developmental stages.</p><p><b>METHODS</b>This study was conducted in 3?, 7?, 14? and 21?day?old SD rats (40 in each age group). In each group, the rats were randomized equally into control group and experimental group, and in the control group, the rats received an intraperitoneal injection of 25 mg/kg medium?long?chain fat emulsion followed by injections at a half dose every 20 min for 8 h; the rats in the experimental group were given injections of propofolmedium (at the initial dose of 25 mg/kg) in the same manner. The transcriptional levels of MBP and caspase?3 in the brain tissues were detected by qRT?PCR, and the protein expression of MBP was with Western blotting and immunehistochemistry.</p><p><b>RESULTS</b>Compared with those in the control groups, the expression of MBP mRNA was significantly down?regulated while caspase?3 mRNA was up?regulated in 3?, 7? and 14?day?old rats in the experimental groups (P<0.05). The protein expression of MBP in 7? and 14?day?old rats was significantly decreased in the experimental groups compared with the control groups (P<0.05). The expression of caspase?3 mRNA or MBP protein in 21?day?old rats showed no significant difference between the two groups (P>0.05).</p><p><b>CONCLUSION</b>Propofol can down?regulate the expression of MBP at both the mRNA and protein levels in SD rats, especially in those at 7 and 14 days of age.</p>
ABSTRACT
Objective To study children′s social adaptive capability and their relative factors.Methods Five hundred and twenty-seven children in 4 different kindergartens were surveyed by using the social adaptive capability-measuring list from infant to junior school student which revised by ZUO Qi-hua and questionnaire of family situation which compiled by TAO Gong-min and Zhu Xiao-qin.Results Twenty-five point three percent of the surveyed children′s social adaptive capabilities were higher than normal level.Sixty-eight point nine percent of them were at normal level.Four point seven percent of them were at edge level.The rest were at a slightly lower level.The influencing factors included the educational level of parents,the educational manner of the parents and their marriage relationship.Conclusions It is necessary to do some investigation to find problem eariler on children′s social adaptive capability.Suitable educational methods and good family environment are important to improve children′s social adaptive capability.